PT  - JOURNAL ARTICLE
AU  - C J Bax
AU  - K D Quint
AU  - R P H Peters
AU  - S Ouburg
AU  - P M Oostvogel
AU  - J A E M Mutsaers
AU  - P J Dörr
AU  - S Schmidt
AU  - C Jansen
AU  - A P van Leeuwen
AU  - W G V Quint
AU  - J B Trimbos
AU  - C J L M Meijer
AU  - S A Morré
TI  - Analyses of multiple-site and concurrent <em>Chlamydia trachomatis</em> serovar infections, and serovar tissue tropism for urogenital versus rectal specimens in male and female patients
AID  - 10.1136/sti.2010.048173
DP  - 2011 Oct 01
TA  - Sexually Transmitted Infections
PG  - 503--507
VI  - 87
IP  - 6
4099  - http://sti.bmj.com/content/87/6/503.short
4100  - http://sti.bmj.com/content/87/6/503.full
SO  - Sex Transm Infect2011 Oct 01; 87
AB  - Objectives The aims of this study were: to determine the incidence of concurrent infections on a serovar level; to determine the incidence of multiple anatomical infected sites on a detection and genotyping level and analyse site-specific serovar distribution; to identify tissue tropism in urogenital versus rectal specimens.Methods Chlamydia trachomatis-infected patients in two populations were analysed: 75 visiting the outpatient department of obstetrics and gynaecology of the MC Haaglanden, and 358 visiting the outpatient sexually transmitted disease clinic, The Hague, The Netherlands. The PACE 2 assay (Gen-Probe) was used to detect C trachomatis from urethral, cervical, vaginal, oropharyngeal and anorectal swabs. C trachomatis genotyping was performed on all C trachomatis positive samples, using the CT-DT genotyping assay.Results Samples from 433 patients (256 female and 177 male) with confirmed C trachomatis infection were analysed. In 11 patients (2.6%), concurrent serovars in one anatomical sample site were present. In 62 (34.1%) female and four (9.3%) male patients, multiple sample site infections were found. A substantial percentage of women tested at the cervical/vaginal and rectal site were found to be positive at both sites (36.1%, 22/61). In men, D/Da and G/Ga serovars were more prevalent in rectal than urogenital specimens (p=0.0081 and p=0.0033, respectively), while serovar E was more prevalent in urogenital specimens (p=0.0012).Conclusions The prevalence of multiple serovar infections is relatively low. Significant differences in serovar distribution are found in rectal specimens from men, with serovar G/Ga being the most prominent, suggesting tissue tropism.