RT Journal Article
SR Electronic
T1 P3.447 Leukocyte Extract Modulates Cellular Factors Involved in HIV Infection
JF Sexually Transmitted Infections
JO Sex Transm Infect
FD BMJ Publishing Group Ltd
SP A288
OP A288
DO 10.1136/sextrans-2013-051184.0897
VO 89
IS Suppl 1
A1 C B Fernandez-Ortega
A1 D Casillas
A1 M Dubed
A1 L Navea
A1 A C Ramirez
A1 L Lopez
A1 T Paneque
YR 2013
UL http://sti.bmj.com/content/89/Suppl_1/A288.2.abstract
AB Background Despite the therapeutic advances of antiretroviral, problems of drug resistance, latent viral reservoirs, and drug induced toxic effects that compromise effective viral control point to the need for new classes of anti-HIV drugs with different modes of action. An extract from human leukocyte called Dialyzable Leukocyte Extract (DLE) delays AIDS progression. However, the molecular bases supporting this effect are not completely comprehended. We hypothesises that active molecules from DLE modulate cellular factors involved in HIV infection. Methods Using an in vitro infection model on MT4 cell line we demonstrated an inhibitory effect of DLE on HIV replication in treated cells which led us to identify molecules that could mediate this and other effects observed in this preparation. Results DLE shows a significant inhibitory effect on HIV replication ranged from 80–90% according to the viral challenge. In addition, DLE shows modulation of important endogenous factors involved in HIV immunopathogenesis like TNFalpha and transcription factors NFkB and Sp1. Conclusions DLE effect on cellular factors involved in HIV replication correlates with DLE inhibitory effect on HIV in vitro replication. The inhibition of HIV replication observed with DLE treatment could be mediated by TNF and transcriptions factors modulation involved in HIV infection. These finding could support the use of DLE on HIV patients and/or in health individuals to prevent the infection.