RT Journal Article
SR Electronic
T1 Observation of a cytopathogenic effect on cell lines used for routine viral cultures led to the diagnosis of lymphogranuloma venereum
JF Sexually Transmitted Infections
JO Sex Transm Infect
FD BMJ Publishing Group Ltd
SP 395
OP 397
DO 10.1136/sextrans-2012-050785
VO 89
IS 5
A1 Busson, Laurent
A1 Crucitti, Tania
A1 De Foor, Marc
A1 Van den Wijngaert, Sigi
A1 Vandenberg, Olivier
YR 2013
UL http://sti.bmj.com/content/89/5/395.abstract
AB Objectives This article reports the fortuitous recovery of nine Chlamydia trachomatis serovar L strains in cell cultures (Vero and LLC-MK2 cell line) designed for viral culture. Methods Nine ano-genital swabs were inoculated on confluent Vero, MRC5 and LLC-MK2 cell cultures. They were collected from HIV-positive patients who were primarily men who have sex with men (MSM) presenting ulcerations that mimicked herpes simplex infections. Results A cytopathogenic effect was observed on Vero and LLC-MK2 cells on day 14. The presence of C trachomatis serovar L in the cell lines was confirmed by Real Time–PCR. Conclusions C trachomatis serovar L can grow on Vero and LLC-MK2 cell lines designed for viral cultures. Lymphogranuloma venereum must be considered as a differential diagnosis for herpes-like lesions, particularly in MSM with high-risk behaviours.