PT - JOURNAL ARTICLE AU - Xu, Wenqi AU - Liu, Jingwei AU - Zhu, Xiaoyu AU - Yin, Yueping TI - P851 Performance disk diffusion method in identifying gonococcal resistance AID - 10.1136/sextrans-2019-sti.893 DP - 2019 Jul 01 TA - Sexually Transmitted Infections PG - A356--A356 VI - 95 IP - Suppl 1 4099 - http://sti.bmj.com/content/95/Suppl_1/A356.2.short 4100 - http://sti.bmj.com/content/95/Suppl_1/A356.2.full SO - Sex Transm Infect2019 Jul 01; 95 AB - Background Because of their simple and cost-effective characteristics, many products of disk diffusion tests are commanly used by microbiological laboratories and hospitals world-wide to determine the gonococcal susceptibility and guide clinical treatments. To evaluate the reliability of routine detection of antimicrobial susceptibility of Neisseria gonorrhoeae by disk diffusion method, six kinds of discs targeted at the specific antibiotics (penicillin, ceftriaxone, cefixime, tetracycline, ciprofloxacin and spectinomycin) from two domestic and one foreign manufacturer were included into the evaluation using agar dilution method as a reference method to validate the concordance between the two methods.Methods According to CLSI 2018 (M100, 28th ed) standard, the antimicrobial sensitivity results of more than 100 clinical isolates and 6 reference strains were interpreted. The coincidence rates of the two methods were analyzed by calculating four indicators: categorical agreement (CA), very major errors (VME), major errors (ME), minus errors (MIE). In addition, the correlation between agar dilution and disk diffusion methods were analyzed by using breakpoints established by linear least-squares regression.Results The results showed that the MIC values of the 6 antibiotics on reference strains determined by the two methods were within the reference ranges. No very major or major discrepancies were detected in the 6 antibiotics. The performance of disk diffusion method to detect gonococcal susceptibility indicted that 1) the coincidence rates in spectinomycin and ciprofloxacin were high (CA≥98%); 2) disk dilution method could miss some penicillin-resistant strains; (3) the coincidence rate of ceftriaxone and cefixime was low (CA<80%), in which agar dilution method is needed to confirm the detection of non-sensitive strains.Conclusion The domestic products for ciprofloxacin, tetracycline, spectinomycin, cefixime and ceftriaxone were as satisfactory as foreign ones but the domestic for penicillin was relatively poor in quality.Disclosure No significant relationships.