PT  - JOURNAL ARTICLE
AU  - Marianne Gossé
AU  - Hilde Lysvand
AU  - Brita Pukstad
AU  - Svein Arne Nordbø
TI  - P608 Simpleprobe PCR assay for detection of mutations associated with macrolide resistance in <em>mycoplasma genitalium samples</em>
AID  - 10.1136/sextrans-2019-sti.676
DP  - 2019 Jul 01
TA  - Sexually Transmitted Infections
PG  - A269--A269
VI  - 95
IP  - Suppl 1
4099  - http://sti.bmj.com/content/95/Suppl_1/A269.2.short
4100  - http://sti.bmj.com/content/95/Suppl_1/A269.2.full
SO  - Sex Transm Infect2019 Jul 01; 95
AB  - Background Macrolide-resistant strains of Mycoplasma genitalium are an increasing problem throughout the world, and the implementation of a rapid and sensitive assay for mutation detection to guide treatment is needed. Macrolide-resistant strains have been shown to contain base substitutions in positions 2058 and 2059 (Escherichia coli numbering) in region V of the 23S rRNA gene. In this study, we present a SimpleProbe PCR followed by melting curve analysis to differentiate between macrolide-resistant mutants and wild types.Methods The assay was performed on 159 Mycoplasma genitalium-positive samples, and the results were compared with DNA sequencing. We also looked at the prevalence of macrolide-resistant strains in a Norwegian population.Results Of 139 samples characterized successfully by sequencing, 54 (39%) were wild types and 85 (61%) were mutants, consisting of 59 (42%) A2059G, 24 (17%) A2058G, 1 (1%) A2058T, and 1 (1%) A2059C mutation. The melting curve analysis correctly differentiated between wild-type and mutant strains in all cases, but it could not identify the different mutant types.Conclusion The SimpleProbe PCR proved to be a simple, rapid, and reliable method for the detection of macrolide-resistant isolates of Mycoplasma genitalium in a clinical setting.Disclosure No significant relationships.