Polymerase chain reaction primers for the detection of Mycoplasma genitalium, Ureaplasma urealyticum, and Trichomonas vaginalis
| Organism | Target | Primers | Sequence |
|---|---|---|---|
| Mycoplasma genitalium | 140 kDa adhesin gene | outer primers | |
| MG PA1 | TTGATGAAACCTTAACCCCTTGG | ||
| MG PA3 | GTTGAGGGGTTTTCCATTTTTGC | ||
| inner primer used with MG PA1 | |||
| MG PA2 | GACCATCAAGGTATTTCTCAACAGC | ||
| Ureaplasma urealyticum | urease gene | UU 9A | AGGAGATAATGATTATATGTCAGG |
| UU 4AR | CGAAACGACGTCCATAAGCAAC | ||
| Trichomonas vaginalis | 650 bp repetitive sequence | outer primers | |
| TVrep1 | AATTGGAACCAGTGTCAGAATAC | ||
| TVrep2R | CCCATTCTTTTAGACCCTTCAG | ||
| inner primer used with TVrep2R | |||
| TVrep3 | ATACAAAACATCCCCAACATCTT |