Volumes (μl) of the constituents of the PCR mix, and amplification procedure used for each of the evaluated PCR assay
| Primer set | Total volume | 10 mM TRIS-HCl (pH 8.3) | 25 mM MgCl2 | 2 mM dNTP | 5 μM primer | 0.5 Units Taq | Milli-Q water* | Sample extract |
|---|---|---|---|---|---|---|---|---|
| *Milli-Q water = distilled water, PCR grade (Millipore, Brussels, Belgium). | ||||||||
| TVK3/7 | 50 | 5 | 4 | 7 | 1.5 | 0.4 | 20.6 | 10 |
| TVA5/6 | 50 | 5 | 5 | 6 | 1 | 0.2 | 11.8 | 20 |
| IP1/2 | 20 | 2 | 1.6 | 2 | 2 | 0.1 | 5.3 | 5 |
| BTUB9/2 | 50 | 5 | 5 | 6 | 1 | 0.5 | 11.5 | 20 |
| TV1/2 | 50 | 5 | 5 | 5 | 4 | 0.25 | 16.75 | 10 |