Table 2

Amplification programmes

Primer setPre-denaturationDenaturationAnnealingExtensionFinal extensionNo of cycles
*Touchdown method: the annealing temperature began at 62°C and was lowered 1 degree. every four cycles until 52°C was reached.
TVK3/795°C, 5 minutes90°C, 60 seconds60°C, 30 seconds70°C, 120 seconds72°C, 7 minutes35
TVA5/695°C, 5 minutes94°C, 60 seconds47°C, 60 seconds67°C, 60 seconds72°C, 7 minutes.35
IP1/295°C, 5 minutes94°C, 45 seconds45°C, 60 seconds72°C, 60 seconds.72°C, 7 minutes.35
BTUB9/295°C, 5 minutes95°C, 45 seconds62°C–52°C, 45 seconds*72°C, 60 seconds.72°C, 7 minutes.60
TV1/295°C, 5 minutes94°C, 10 seconds58°C, 45 seconds72°C, 15 seconds.72°C, 7 minutes.40