Table 1 Oliogonucleotides used in the real-time quadriplex PCR assay

Target	Primer or probe	Nucleotide sequence (5′ to 3′)	Final concentration (nmol)
pmpH	Forward primer	GGATAACTCTGTGGGGTATTCTCCT	600
pmpH	Reverse primer	AGACCCTTTCCGAGCATCACT	600
pmpH	LGV-specific probe	VIC-CCTGCTCCAACAGT–MGB	200
pmpH	Non-LGV specific probe	FAM-GCTTGAAGCAGCAGGAGCTGGTG–BHQ	200
Plasmid	Forward primer	GGATTGACTCCGACAACGTATTC	50
Plasmid	Reverse primer	ATCATTGCCATTAGAAAGGGCATT	100
Plasmid	Probe	CalRed610–TTACGTGTAGGCGGTTTAGAAAGCGG–BHQ	100
RNase P	Forward primer	CCAAGTGTGAGGGCTGAAAAG	80
RNase P	Reverse primer	TGTTGTGGCTGATGAACTATAAAAGG	80
RNase P	Probe	CY5–CCCCAGTCTCTGTCAGCACTCCCTTC–BHQ	80