Table 2 Comparison between real-time duplex and quadriplex PCR assay results
Duplex
LGVNon-LGVNegativeTotal
Quadriplex
    LGV34*0034
    Non-LGV035035
    Negative03†1619
    Mixed01‡01
    Total34391689
  • *33/34 confirmed as L2 by the Health Protection Agency (HPA).

  • †Two specimens gave equivocal results by the duplex assay and all three were positive only for plasmid target and human DNA control in the quadriplex assay.

  • ‡Identified as lymphogranuloma venereum (LGV) by the HPA.