Table 1 Patient demographics and distribution of Haemophilus ducreyi (HD), Treponema pallidum (TP), herpes simplex virus type 1 (HSV-1) and HSV-2 by multiplex real-time PCR

	Genital ulcer disease aetiology by real-time PCR
	HDn (%)	TPn (%)	HSV-1n (%)	HSV-2n (%)	HD/HSV-2 n (%)	NDn (%)
Total (n = 100)	1 (1)	5 (5)	3 (3)	61 (61)	1 (1)	29 (29)
Gender
Male (n = 50)	1 (2)	2 (4)	3 (6)	28 (56)	1 (2)	15 (30)
Female (n = 50)	0 (0)	3 (6)	0 (0)	33 (66)	0 (0)	14 (28)
Age (y)
19–29 (n = 51)	0 (0)	3 (6)	0 (0)	33 (65)	1 (2)	14 (27)
30–39 (n = 31)	0 (0)	1 (3)	0 (0)	20 (65)	0 (0)	10 (32)
40+ (n = 15)	0 (0)	0 (0)	3 (20)	7 (47)	0 (0)	5 (33)
Not known (n = 3)	1 (33)	1 (33)	0 (0)	1 (33)	0 (0)	0 (0)
HIV serostatus
Negative (n = 57)	1 (2)	4 (7)	2 (4)	29 (51)	1 (2)	20 (35)
Positive (n = 43)	0 (0)	1 (2)	1 (2)	32 (74)	0 (0)	9 (21)

ND, pathogen not detected by real-time PCR.