Table 2

NG-MAST results for the 46 N gonorrhoeae PCR-positive clinical sample DNA extracts tested at QPID-Bris

Sample no	Site of collection	NG-MAST			Ct value
Sample no	Site of collection	porB	tbpB	Sequence type	(NG duplex PCR)
1	Urine	4	4	225	22
2	Urine	187	33	251	22
3	Cervical swab	767	26	1182	24
4	Urine	1090 (99%)	330	new	24
5	Urine	461	270 (95%)	new	24
6	Cervical swab	904	349	1415	25
7	Urine	4	4	225	25
8	Urine	904	349	1415	25
9	Urine	904	349	1415	25
10	Urine	147	137	1797	25
11	Rectal swab	262	4	757	25
12	Urine	262	4	757	26
13	Urine	147	91	1479	26
14	Urine	95 (99%)	33 (99%)	New	26
15	Rectal swab	59	16	691	26
16	Urethral swab	4	4	225	27
17	Cervical swab	53	122	341	28
18	Urethral swab	4 (99%)	4	225	28
19	Urine	4 (99%)	4	225	28
20	Urine	4	4	225	28
21	Urine	467 (99%)	4	778	28
22	Urine	53	21	147	29
23	Urine	904	349	1415	29
24	Rectal swab	30	35	40	29
25	Rectal swab	59	16	691	29
26	Rectal swab	250	4	323	30
27	Cervical swab	922	355	1431	31
28	Urine	4	4	225	31
29	Urine	4	4	225	31
30	Urine	61	27	356	31
31	Rectal swab	4	4	225	31
32	Rectal swab	Not available	Not available	Not available	31
33	Cervical swab	171	27	1485	32
34	Cervical swab	581 (99%)	28	New	32
35	Vaginal swab	904	349	1415	32
36	Urethral swab	4	4	225	33
37	Throat swab	59	16	691	33
38	Urine	370	21	New	34
39	Cervical swab	262	29	New	35
40	Rectal swab	912	33	1419	35
41	Throat swab	4	4	225	35
42	Rectal swab	Not available	Not available	Not available	36
43	Throat swab	167 (99%)	223 (97%)	New*	36
44	Throat swab	4	Not available	Not available	37
45	Throat swab	Not available	85	Not available	39
46	Throat swab	Not available	Not available	Not available	40

Ct values obtained using the diagnostic NG duplex PCR in the previous study are also provided.
New, a matching sequence type could not be assigned by the NG-MAST database and were considered to represent previously unidentified alleles or combinations of alleles.
Not available, PCR of amplification of porB, tbpB or both sequences was unsuccessful for these specimens.
↵* These sequences were suspected to be from non-gonococcal Neisseria species based on Genbank Blast.
Ct, cycle threshold; NG, N gonorrhoeae; NG-MAST, Neisseria gonorrhoeae multi-antigen sequence typing; QPID-Bris, Queensland Paediatric Infectious Diseases Laboratory located in Brisbane, Queensland.