Details of C trachomatis test results for the 13 discordant specimens
| No of specimens | LDQA screen | COBAS screen | Additional tests | ‘Resolved’ CT result | ||
| LDQA CT cryptic plasmid | LDQA CT MOMP | LDQA CT result | COBAS CT Taqman | Artus CT Plus | ||
| Cryptic plasmid | cryptic plasmid and MOMP | |||||
| (# female) | (CP range) | (CP range) | (CP range) | |||
| 1 (0) | >45 | − | CT DNA detected | − | Inhibitory | − |
| 2 (1) | 34–42 | 36–39 | CT DNA detected | − | 33–36 | CT positive |
| 1 (0) | − | − | − | CT DNA detected | Inhibitory | − |
| 1 (1) | 43 | − | CT DNA detected | CT DNA detected | − | CT positive |
| 1 (1) | 41 | − | CT DNA detected | Inhibitory | 37 | CT positive |
| 1 (0) | 39 | 40 | CT DNA detected | CT DNA detected | − | CT positive |
| 4 (1) | 36–40 | − | CT DNA detected | CT DNA detected | 34–37 | CT positive |
| 2 (0) | − | − | − | Inhibitory | Inhibitory | Unclear* |
| Total 13 (4) | ||||||
Results for all other specimens were concordant. Resolved results were used in laboratory-developed quadruplex assay (LDQA) kappa analysis.
−, Negative; CP, real-time PCR crossing point; CT, C trachomatis.
Results for the remaining 79 CT resolved positives were concordant.
↵* Two samples were excluded from the CT kappa analysis due to repeatedly inhibitory results.