Evaluation of the operational performance of the test based on five characteristics of the RT
| Assay | Operational assay performance score | |||||
| No. of reagents | Reagent storage temperature | No. of steps | Total execution time (hh:mm)* | Technical skills of operator | Assay performance | |
| Determine Syphilis TP | 1 (1) | 1 (2–30°C) | 1 (3) | 1 (00:16) | 1 | Good (5/5) |
| Imuno-Rápido Syphilis | 1 (1) | 1 (2–30°C) | 1 (3) | 1 (00:11) | 1 | Good (5/5) |
| Rapid-Check Syphilis | 1 (1) | 1 (5–30°C) | 1 (3) | 1 (00:11) | 1 | Good (5/5) |
| SyphilisTest Biocon | 1 (1) | 1 (2–30°C) | 1 (3) | 1 (00:11) | 1 | Good (5/5) |
| Syphilis Strip Test Bioeasy | 1 (1) | 1 (2–30°C) | 1 (3) | 1 (00:21) | 1 | Good (5/5) |
| RT ABON | 1 (1) | 1 (2–30°C) | 1 (3) | 1 (00:11) | 1 | Good (5/5) |
| RT DPP Syphilis | 0 (2) | 1 (2–30°C) | 1 (4) | 1 (00:16) | 1 | Good (4/5) |
*Total execution time considered to be the time taken between the placement of the samples and the buffer (s) in the test device, plus the maximum time allowed for reading the result. The time taken to obtain the sample and prepare the work area for the test was not considered.
RT, rapid test.