Interassay and intra-assay reproducibility of NT-MAA
| Specimen | Dilutions | RPRa | NT-MAA | ||||||||||||||
| Intra-assay | Interassay | ||||||||||||||||
| No of test repeats | No of assay repeats | ||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 | 5 | |||
| BS1505/RPR reactive specimen | Neat (1:1) | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R |
| 1:2 | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | |
| 1:4 | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | |
| 1:8 | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | |
| 1:16 | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | |
| 1:32 | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | R | |
| 1:64 | Rm | Rm | >Rm | Rm | Rm | Rm | Rm | Rm | >Rm | Rm | Rm | Rm | Rm | Rm | Rm | Rm | |
| 1:128 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| 1:256 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| 1:512 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| RPR N control | Neat (1:1) | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N |
N, non-reactive; NT-MAA, non-treponemal magnetic particle-based agglutination assay; R, reactive; Rm, minimal reactivity; >Rm, slightly more reactivity than minimal reactive; RPRa, rapid plasma reagin (semiquantitative).