Table 1

Results for macrolide and/or fluoroquinolone resistance in Mycoplasma genitalium isolates (N=69)

Study sites (location) (N=69)Samples (n)Average Ct value in M. genitalium PCRSpeeDx ResistancePlus assay
Macrolide resistance
TaqMan S83I and S83 assay
Fluoroquinolone resistance
Summary
23SRNA mutationWild-type*S83I mutant
Site 1 (rural), n=171534.8Not detectedDetectedNot detectedCharacterised
139.9Not detectedNo amplificationNo amplificationPartially characterised
138.5No amplificationNo amplificationNo amplificationNot characterised
Site 2 (urban), n=11635.5Not detectedDetectedNot detectedCharacterised
137.9Not detectedNo amplificationNo amplificationPartially characterised
438.9No amplificationNo amplificationNo amplificationNot characterised
Site 3 (rural), n=11836.4Not detectedDetectedNot detectedCharacterised
238.5Not detectedNo amplificationNo amplificationPartially characterised
138.5No amplificationNo amplificationNo amplificationNot characterised
Site 4 (urban), n=11836.8Not detectedDetectedNot detectedCharacterised
139.2Not detectedNo amplificationNo amplificationPartially characterised
134.2No amplificationDetectedNot detectedPartially characterised
136.5No amplificationNo amplificationNo amplificationNot characterised
Site 5 (rural), n=191636.1Not detectedDetectedNot detectedCharacterised
339.5No amplificationNo amplificationNo amplificationNot characterised
  • Detected: sample was tested and we achieved an evaluable result (eg, no macrolide resistance observed and/or fluoroquinolone susceptibility was confirmed). No amplification: sample was tested; however, no evaluable result was obtained. Characterised: sample was successfully characterised by both assays. Not characterised: sample was not evaluable by both assays. Partially characterised: sample was successfully characterised by one assay.

  • *Wild-type (S83 sequence, no mutation) indicates fluoroquinolone susceptibility.