Abstract
Two simple, inexpensive media containing gonococcal agar-base, supplemented with 5 % Fildes' extract and either chocolated or unchocolated horse blood (GC-FHBC or GC-FHB) were compared with the standard gonococcal agar-based (GC-HgS) and Mueller-Hinton agar-based media (MH-HB) normally used for primary isolation ofHaemophilus ducreyi from presumptive chancroid lesions. Overall,Haemophilus ducreyi was recovered from 162 of 178 (91 %) samples from primary chancroid lesions. As a single isolation medium GC-HgS proved the most sensitive with an isolation rate of 80 % followed by GC-FHB (75 %), MH-HB and GC-FHC (both 71 %). Use of a combination of GC-HgS and MH-HB resulted in isolation ofHaemophilus ducreyi in 160 of 178 cases (90 %). Since GC-FHB is approximately one-quarter the cost of the combination and half the cost of GC-HgS or MH-HB alone, this medium could prove suitable for diagnostic purposes in developing countries where chancroid is endemic.
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Dangor, Y., Miller, S.D., Koornhof, H.J. et al. A simple medium for the primary isolation ofHaemophilus ducreyi . Eur. J. Clin. Microbiol. Infect. Dis. 11, 930–934 (1992). https://doi.org/10.1007/BF01962377
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DOI: https://doi.org/10.1007/BF01962377