Abstract
The presence ofHelicobacter pylori in the oral cavity (6 sites), oesophagus, stomach and bowel of 20 dyspeptic patients was investigated. Samples were cultured on three selective media and analyzed by 16S rDNA polymerase chain reaction (PCR) and southern hybridization.Helicobacter pylori DNA was detected by PCR from oral-cavity samples of three (20 %) and from faeces samples of only one (7 %) of the patients whose stomach biopsies were positive forHelicobacter pylori. When culture was used, the microorganism's rate of recovery from the oral cavity and faeces was 13 % and 7%, respectively. One patient had aHelicobacter pylori-like organism in samples collected from the tongue and palate. Both strains were urease, catalase and oxidase positive and grew microaerophilically but were negative on PCR analysis. This demonstrates the possibility of false identification ofHelicobacter pylori by use of routine enzyme reactions. Interestingly, specimens collected from the cheeks of three patients were positive forHelicobacter pylori by PCR analysis. This is the first instance of detection of this micro-organism in the cheek.
Similar content being viewed by others
References
Marshall BJ, Warren JR: Unidentified curved bacilli in the stomach of patients with gastric and peptic ulceration. Lancet 1984, i: 1311–1315.
Rauws EAJ, Tytgat GJN: Cure of duodenal ulcer associated with eradication ofHelicobacter pylori. Lancet 1990, 335: 1233–1235.
Syed SA, Loesche WJ: Survival of human dental plaque flora in various transport media. Applied Microbiology 1972, 24: 638–644.
Skirrow MB:Campylobacter enteritis: a new disease. British Medical Journal 1977, 2: 9–11.
Dent JC, McNulty CAM: Evaluation of a new selective medium forCampylobacter pylori. European Journal of Clinical Microbiology & Infectious Diseases 1988, 7: 555–568.
Queiroz DMM, Mendes EN, Rocha GA: Indicator medium for isolation ofCampylobacter pylori. Journal of Clinical Microbiology 1987, 25: 2378–2379.
Thomas JE, Gibson GR, Darboe MK, Dale A, Weaver LT: Isolation ofHelicobacter pylorifrom human faeces. Lancet 1992, 340: 1194–1195.
Roosendaal R, Kuipers EJ, Van Den Brule AJC, Peña AS, Uyterlinde AM, Walboomers JMM, Meuwissen SGM, De Graaff J: Importance of the fiberoptic endoscope cleaning procedure for detection ofHelicobacter pylori in gastric biopsy specimens by PCR. Journal of Clinical Microbiology 1994, 32: 1123–1126.
Maniatis T, Fritsch TEF, Sambrook J: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982.
Khakoo SI, Lobo AJ, Shepherd NA, Wilkinson SP: Histological assessment of the Sydney classification of endoscopic gastritis. Gut 1994, 35: 1172–1175.
Van Zwet AA, Thijs JC, Kooistra-Smid AMD, Snijder JAM: Use of PCR with feces for detection ofHelicobacter pylori infection in patients. Journal of Clinical Microbiology 1994, 32: 1346–1348.
Shimada B, Ogura K, Ota S, Terano A, Takahashi M, Hamada E, Omata M, Sumino S, Sassa R: Identification ofHelicobacter pylori in gastric specimens, gastric juice, saliva and faeces of Japanese patients. Lancet 1994, 343: 1636–1637.
Mapstone NP, Lynch DAF, Lewis FA, Axon ATR, Tompkins DS, Dixon MF, Quirke P: PCR identification ofHelicobacter pylori in faeces from gastritis patients. Lancet 1993, 341: 447.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Namavar, F., Roosendaal, R., Kuipers, E.J. et al. Presence ofHelicobacter pylori in the oral cavity, oesophagus, stomach and faeces of patients with gastritis. Eur. J. Clin. Microbiol. Infect. Dis. 14, 234–237 (1995). https://doi.org/10.1007/BF02310363
Issue Date:
DOI: https://doi.org/10.1007/BF02310363