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Clinical evaluation of commercial PCR assays for antimicrobal resistance in Mycoplasma genitalium and estimation of resistance-mediated mutation prevalence in Moscow and Moscow region

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Abstract

Mycoplasma genitalium is a widespread sexually transmitted infection (STI) with growing rate of antimicrobials resistance. In our study, 137 vaginal and 131 urethral M. genitalium–positive swabs were sequentially collected through the work of Reference Center for STI during 2019. For prevalence evaluation of macrolide-resistance mutations three commercially available kits were used: AmpliSens® M. genitalium-ML/FQ-Resist-FL (Central Research Institute of Epidemiology, Russia), ResistancePlus® MG (SpeeDx, Australia), and S-DiaMGRes™ (Diagenode, Belgium). Macrolide resistance mutations were detected in 16% (43 of 268) of samples. Diagnostic characteristics were evaluated against Sanger sequencing. For AmpliSens® M. genitalium-ML/FQ-Resist-FL specificity was shown to be 100% (CI 95%, 98.4–100), and sensitivity was 90.7% (CI 95%, 77.9–97.4). ResistancePlus® MG specificity was 100% (CI 95%, 98.3–100), and sensitivity was 92.1% (CI 95%, 78.6–98.3). S-DiaMGRes™ specificity was shown to be 88.6% (CI 95%, 83.9–92.4), and sensitivity was 100% (CI 95%, 84.4–100). Mutations of parC gene region were detected in 14.5% (38 of 268) using AmpliSens® M. genitalium-ML/FQ-Resist-FL with further validation by Sanger sequencing. Of studied samples, 6.3% (17 of 268) contained both antimicrobials of class resistance mutations. Prevalence of macrolide-resistant M. genitalium in Moscow was 21.7% (23 of 106) and of fluoroquinolone-resistant M. genitaliuim was 20.8% (22 of 106). In Moscow region, macrolide-resistant M. genitalium were 12.3% (20 of 162) and 9.9% (16 of 162) of fluoroquinolone-resistant M. genitalium. All three kits can be used both for epidemiological monitoring of M. genitalium presence and mutation prevalence estimation. In Moscow, macrolide- and fluoroquinolone-resistant mutant prevalence increased in 3.9 and 2.7 times in 3 years.

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Authors

Contributions

Study concept and design—Shedko E.D., Khayrullina G.A., Goloveshkina E.N.

Collection and processing of material—Shedko E.D., Khayrullina G.A.

Statistical analysis—Shedko E. D.

Writing—Shedko E.D.

Editing—Goloveshkina E.N., Akimkin V.G.

Approval of article final version—all co-authors.

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Correspondence to Shedko Elizaveta Dmitrievna.

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Shedko, E.D., Khayrullina, G.A., Goloveshkina, E.N. et al. Clinical evaluation of commercial PCR assays for antimicrobal resistance in Mycoplasma genitalium and estimation of resistance-mediated mutation prevalence in Moscow and Moscow region. Eur J Clin Microbiol Infect Dis 40, 1413–1418 (2021). https://doi.org/10.1007/s10096-021-04170-0

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  • DOI: https://doi.org/10.1007/s10096-021-04170-0

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