Original researchNugent score related to vaginal culture in pregnant women1☆,
Section snippets
Materials and methods
This study was conducted as part of a larger project collecting quantitative data on the microbiologic population in pregnant women in two high-risk cohorts for preterm delivery (prior preterm birth and bleeding during the current pregnancy) and a low-risk cohort (no prior preterm birth or bleeding) to formulate a model to determine the bacteriologic factors promoting preterm labor. Eligible women were identified by the study nurse and approached during routine antenatal care visits at the
Results
Of the 104 smears examined using the Nugent criteria, 71 (68%) were graded as normal, 22 (21%) as intermediate, and 11 (11%) as BV. The mean counts for the individual components comprising the Nugent score were determined. The mean count for Lactobacillus was 9.08 ± 0.50 log10 cfu/g, 8.81 ± 0.80 log10 cfu/g, 8.55 ± 0.84 log10 cfu/g, 8.75 ± 0.58 log10 cfu/g, and 8.30 ± 0.80 log10 cfu/g for a Nugent score of 0, 1, 2, 3, and 4, respectively. The gram-variable and gram-negative score comprised G
Discussion
The vaginal microflora associated with BV during pregnancy has been evaluated by a number of investigators.12, 13, 14, 15, 16 The methods used in these studies to assess the bacterial load have included vaginal swabs diluted in transport media or vaginal wash samples plated directly onto various media. None of these methods provide a denominator for comparing the bacterial counts. In vaginal wash samples, an inherent variability exists because vaginal fluids are randomly diluted depending on
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Cited by (62)
Crosstalk between Vaginal Microbiome and Female Health: A review
2019, Microbial PathogenesisCitation Excerpt :As a consequence of the prolonged use of antibiotics, Peptostreptococcus anaerobius and Gardnerella vaginalis, Atopobium vaginae, Mobiluncus sp. Sneathia, Prevotella sp., Mycoplasma, Ureaplasma, and numerous uncultivated or fastidious anaerobes become more active and can cause BV and other associated disorders due to the decrease in the levels of Lactobacillus [45,46]. Thus, the health status of a female can be predicted by observing the composition of the vaginal microbiome.
Cervicovaginal Microbiota and Reproductive Health: The Virtue of Simplicity
2018, Cell Host and MicrobeCervicovaginal bacterial count and failure of metronidazole therapy for bacterial vaginosis
2016, International Journal of Gynecology and ObstetricsCitation Excerpt :At present, there is no concrete evidence to support the role of vaginal bacterial composition in the treatment failure of bacterial vaginosis. Although differences have been reported in the number of CFUs, bacterial load, and total bacterial count of cervicovaginal samples between women with bacterial vaginosis and those with normal flora [13–16], the present study did not find differences in the total bacterial count either between women with and those without bacterial vaginosis, or between women who were treated successfully and those who persisted with this condition after metronidazole therapy. Previous studies reported that bacterial vaginosis was associated with increased bacterial diversity [9], loading of a particular genus [15], and higher numbers of CFUs [13,14].
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This study was supported by grant RO1 HD35667 from the National Institutes of Health.
- 1
The Microbiology and Prematurity Study Group consists of the following: Robin Ross, PhD, Mei-Ling Lee, PhD, Andrea M. DuBois, BS, Wendy Osterling, BS, and David G. Aiello, BS, Channing Laboratory, Brigham and Women’s Hospital, Boston, MA; and Ruth Tuomala, MD, Ellice Lieberman, MD, Amy Cohen, BA, Dorothy Pender, RN, and Linda Steele, MT(ASCP), Department of Obstetrics and Gynecology, Brigham and Women’s Hospital, Boston, MA.