Genetics and evolution of Neisseria meningitidis: Importance for the epidemiology of meningococcal disease
Section snippets
Introduction: the meningococcus, a human commensal
Neisseria meningitidis, the meningococcus, is a Gram-negative diplococcus bacterium, member of the family Neisseriaceae (Cimolai and Caugant, 2001). It is an obligate commensal of humans and the majority of people will at one time or another harbor the bacterium asymptomatically in the throat, a phenomenon known as carriage (Broome, 1986). N. meningitidis survives only very shortly in the environment; thus, transmission between hosts requires the spread of upper respiratory secretions from an
Meningococcal disease
Occasionally, shortly after the onset of colonization, N. meningitidis strains might penetrate the mucosal membrane and gain access to the bloodstream. Bacteria that are able to survive and multiply in the circulation may then cross the meninges and gain access to the cerebrospinal fluid. Of the various forms of meningococcal disease, the most common ones are meningitis, which may occur as epidemics, and severe sepsis with often a rapid fatal outcome (Brandtzæg and van Deuren, 2002). Despite
The bacterium and serological classification
A capsular polysaccharide, which is protecting the bacterium during the invasion process, is the outermost antigenic structure on the meningococcal surface and the primary target for mucosal and humoral immunity. Strains of N. meningitidis have been classified since the 1930s by serological typing based on antigenic properties of the capsular polysaccharide, which identifies the serogroup. Twelve serogroups are distinguished and chemically defined: A, B, C, 29E, H, I, K, L, W135, X, Y, and Z.
Genetic methods for the characterization of N. meningitidis
While serological typing methods have been useful for public health decisions in relation to disease control and vaccine development, they exhibit a number of limitations, which had made them inappropriate for epidemiological studies of meningococcal disease. Among these limitations, the most significant ones are: (1) incomplete coverage of typing reagents; (2) typing target not expressed in all isolates; and (3) poor correlation with genetic relatedness of isolates due to the high levels of
Genetic variation in housekeeping genes and population structure
There are two main factors that determine how different microbial organisms derived from a common ancestor are: the time elapsed since their divergence and their potential for evolutionary changes. The potential for evolution of bacteria depends upon the genetic mechanisms that they have available (mutation, recombination, inversion, DNA repair, etc.) and their respective rates as well as upon the structuring and the ecology of their populations. Bacteria that are able to incorporate foreign
Antigenic variation and virulence
In the presence of extensive recombination the persistence of clonal complexes suggests that some important selective forces are maintaining the structuring of the population of N. meningitidis. Factors such as virulence characteristics, transmissibility, and susceptibility of the human reservoir to specific strains or clonal groups will result in limiting the gene flow between meningococcal strains.
A prerequisite for an increased incidence of meningococcal disease in a population is the spread
Challenges for disease control
Even in parts of the world where state of the art treatment can be obtained, meningococcal disease continues to be a frightening disease with often a fatal outcome, because of its rapid onset and progression, particularly in case of sepsis. Effective prevention strategies relying on vaccination have therefore been the focus of much attention. Based on the importance of the capsule for pathogenesis and immunity, capsular polysaccharide vaccines were developed and introduced in the 1970s and
References (106)
- et al.
Definition of meningococcal class 1 OMP subtyping antigens by monoclonal antibodies
FEMS Microbiol. Immunol.
(1988) - et al.
Neisseria meningitidis group B serosubtyping using monoclonal antibodies in whole-cell ELISA
Microb. Pathog.
(1988) - et al.
Effect of outer membrane vesicle vaccine against group B meningococcal disease in Norway
Lancet
(1991) Applications of multilocus enzyme electrophoresis in medical microbiology
J. Microbiol. Methods
(1997)- et al.
Sequence variation in class 1 outer membrane protein in Neisseria meningitidis isolated from patients with meningococcal infection and close household contacts
FEMS Microbiol. Lett.
(1995) - et al.
Transformation-mediated exchange of virulence determinants by co-cultivation of pathogenic neisseriae
FEMS Microbiol. Lett.
(1992) - et al.
Living in a changing environment: insights into host adaptation in Neisseria meningitidis from comparative genomics
Int. J. Med. Microbiol.
(2007) - et al.
Identification of a new Neisseria meningitidis serogroup C clone from Anhui province
China. Lancet
(2006) Uncloaking the meningococcus: dynamics of carriage and disease
Lancet
(1999)- et al.
Epidemic meningitis, meningococcaemia, and Neisseria meningitidis
Lancet
(2007)
Consensus guidelines for appropriate use and evaluation of microbial epidemiological typing systems
Clin. Microbiol. Infect.
Genetic mechanisms for loss of encapsulation in polysialyltransferase-gene-positive meningococci isolated from healthy carriers
Int. J. Med. Microbiol.
Global epidemiology of meningococcal disease
New functional identity for DNA uptake sequence in transformation and its presence in transcriptional terminators
J. Bacteriol.
Dynamics of the meningococcal carrier state and characteristics of the carrier strains: a longitudinal study within three cohorts of military recruits
Epidemiol. Infect.
Sequencing of porA from clinical isolates of Neisseria meningitidis defines a subtyping scheme and its genetic regulation
Can. J. Microbiol.
Randomly amplified polymorphic DNA genotyping of serogroup A meningococci yields results similar to those obtained by multilocus enzyme electrophoresis and reveals new genotypes
J. Clin. Microbiol.
Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18
PLoS Genet.
Meningococcal vaccines
Expert Rev. Vaccines
Applications of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis
J. Clin. Microbiol.
Meningococcal meningitis: unprecedented incidence of serogroup X-related cases in 2006 in Niger
Clin. Infect. Dis.
Current concepts in the role of the host response in Neisseria meningitidis septic shock
Curr. Opin. Infect. Dis.
The carrier state: Neisseria meningitidis
J. Antimicrob. Chemother.
Analysis of the clonal relationships between strains of Neisseria meningitidis by pulsed field gel electrophoresis
J. Gen. Microbiol.
Carriers of Neisseria meningitidis in household contacts of meningococcal disease cases in Catalonia (Spain)
Eur. J. Epidemiol.
The Stonehouse survey: nasopharyngeal carriage of meningococci and Neisseria lactamica
Epidemiol. Infect.
Population genetics and molecular epidemiology of Neisseria meningitidis
APMIS
Genetic diversity and temporal variation in the E. coli population of a human host
Genetics
Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease
Proc. Natl. Acad. Sci. U.S.A.
Genetic structure of populations of Neisseria meningitidis in relation to serogroup, serotype, and outer membrane protein patterns
J. Bacteriol.
Asymptomatic carriage of Neisseria meningitidis in a randomly sampled population
J. Clin. Microbiol.
Lessons from meningococcal carriage studies
FEMS Microbiol. Rev.
Gram negative cocci and Moraxellae
Many carried meningococci lack the genes required for capsule synthesis and transport
Microbiology
Genetics of capsule O-acetylation in serogroup C, W-135 and Y meningococci
Mol. Microbiol.
Genetic analysis of meningococci carried by children and young adults
J. Infect. Dis.
Deletion of the meningococcal fetA gene used for antigen sequence typing of invasive and commensal isolates from Germany: frequencies and mechanisms
J. Clin. Microbiol.
Meningococcal genome dynamics
Nat. Rev. Microbiol.
The meningococcus and mechanisms of pathogenicity
Microbiol. Rev.
Inference of bacterial microevolution using multilocus sequence data
Genetics
Clonal analysis of the serogroup B meningococci causing New Zealand's epidemic
Epidemiol. Infect.
IS1301 fingerprint analysis of Neisseria meningitidis strains belonging to the ET-15 clone
J. Clin. Microbiol.
The relative contributions of recombination and mutation to the divergence of clones of Neisseria meningitidis
Mol. Biol. Evol.
eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data
J. Bacteriol.
Three cases of invasive meningococcal disease caused by a capsule null locus strain circulating among healthy carriers in Burkina Faso
J. Infect. Dis.
An epidemiologically valuable typing method for Neisseria meningitidis by analysis of restriction fragment length polymorphisms
J. Med. Microbiol.
Phase variable type III restriction-modification systems of host-adapted bacterial pathogens
Mol. Microbiol.
Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes
Rev. Infect. Dis.
A universal vaccine for serogroup B meningococcus
Proc. Natl. Acad. Sci. U.S.A.
Case fatality rates for meningococcal disease in an English population, 1963–1998: database study
BMJ
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Neisseria meningitidis
2023, Molecular Medical Microbiology, Third EditionPopulation structure of invasive Neisseria meningitidis in the United States, 2011–15
2018, Journal of InfectionCitation Excerpt :There were 23 STs present in all three time periods comprising 71.0% (2000–05), 75.2% (2006–10) or 69.1% (2011–15) of the population, respectively. Six of the 23 shared STs had more than 20 isolates in at least one time period including ST-11/CC11, ST-23/CC23, ST-32/CC32, ST-136/CC41/44, ST-162/CC162 and ST-2006/CC103, which predominantly correspond to the main hyperinvasive lineages.14 Although there was some variance in CC composition, the common CCs were consistent over time.