Abstract
Polymerase chain reaction (PCR) was used to detect the 47-kDa immunogen gene of Treponema pallidum in peripheral blood mononuclear cells (PBMCs), skin lesions, and serum, but less consistently in purified granulocytes or ejaculates of patients with manifest and latent syphilis. Therefore, skin lesions and PBMCs may serve as the most reliable sources for a PCR-based diagnosis of syphilis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Carrier Proteins / chemistry
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Carrier Proteins / isolation & purification*
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DNA, Bacterial / analysis*
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Female
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Humans
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Lipoproteins / chemistry
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Lipoproteins / isolation & purification*
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Male
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Molecular Sequence Data
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Polymerase Chain Reaction*
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Sensitivity and Specificity
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Skin / microbiology
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Syphilis / diagnosis*
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Syphilis, Cutaneous / diagnosis
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Syphilis, Latent / diagnosis
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Treponema pallidum / genetics*
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Treponema pallidum / isolation & purification*
Substances
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47-kDa immunogen, Treponema pallidum
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Carrier Proteins
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DNA, Bacterial
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Lipoproteins