Lymphogranuloma venereum variant L2b-specific polymerase chain reaction: insertion used to close an epidemiological gap

S P Verweij; A Catsburg; S Ouburg; A Lombardi; R Heijmans; F Dutly; R Frei; S A Morré; D Goldenberger

doi:10.1111/j.1469-0691.2011.03481.x

Lymphogranuloma venereum variant L2b-specific polymerase chain reaction: insertion used to close an epidemiological gap

Clin Microbiol Infect. 2011 Nov;17(11):1727-30. doi: 10.1111/j.1469-0691.2011.03481.x. Epub 2011 Sep 6.

Authors

S P Verweij¹, A Catsburg, S Ouburg, A Lombardi, R Heijmans, F Dutly, R Frei, S A Morré, D Goldenberger

Affiliation

¹ Laboratory of Immunogenetics, Department of Pathology, University Hospital L. Sacco, Milano, Italy.

PMID: 21895856
DOI: 10.1111/j.1469-0691.2011.03481.x

Abstract

The management of the ongoing lymphogranuloma venereum epidemic in industrialized Western countries caused by Chlamydia trachomatis variant L2b still needs improvements in diagnosis, therapy and prevention. We therefore developed the first rapid C. trachomatis variant L2b-specific polymerase chain reaction to circumvent laborious ompA gene sequencing.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Outer Membrane Proteins / genetics
Bacteriological Techniques / methods*
Base Sequence
Chlamydia trachomatis / classification*
Chlamydia trachomatis / genetics
Chlamydia trachomatis / isolation & purification*
Cross Reactions
DNA Primers / genetics
Europe
Humans
Lymphogranuloma Venereum / diagnosis*
Lymphogranuloma Venereum / microbiology*
Male
Molecular Diagnostic Techniques / methods*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sequence Alignment
Sequence Analysis, DNA

Substances

Bacterial Outer Membrane Proteins
DNA Primers
PmpH protein, Chlamydia trachomatis

Associated data

GENBANK/EF534758
GENBANK/EF612788