During routine screening for genital herpes simplex virus infection in patients attending a sexually transmitted diseases clinic adenovirus type 19 was isolated from both men and women. Peak incidences of genital infection with adenovirus type 19 corresponded with those of eye infection with the same virus in the general community. Thus, the relationship between genital and eye infection with adenovirus, the part played by genital infection in its dissemination, and the clinical symptoms it may produce need further study.