Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences

AGCL Speksnijder, GA Kowalchuk… - Applied and …, 2001 - Am Soc Microbiol
ABSTRACT A defined template mixture of seven closely related 16S-rDNA clones was used
in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in …

Genetic Diversity of the Biofilm CoveringMontacuta ferruginosa (Mollusca, Bivalvia) as Evaluated by Denaturing Gradient Gel Electrophoresis Analysis and Cloning of …

DC Gillan, AGCL Speksnijder, G Zwart… - Applied and …, 1998 - Am Soc Microbiol
The shell of the bivalve Montacuta ferruginosa, a symbiont living in the burrow of an
echinoid, is covered with a rust-colored biofilm. This biofilm includes different morphotypes …

Lactobacillus iners-dominated vaginal microbiota is associated with increased susceptibility to Chlamydia trachomatis infection in Dutch women: a case–control study

R Van Houdt, B Ma, SM Bruisten… - Sexually transmitted …, 2018 - sti.bmj.com
Introduction This prospective study aimed to study the composition and structure of the
vaginal microbiota prior to Chlamydia trachomatis infection. Methods A nested case–control …

Anal and penile high-risk human papillomavirus prevalence in HIV-negative and HIV-infected MSM

F Van Aar, SH Mooij, MAB Van Der Sande… - Aids, 2013 - journals.lww.com
Objective: Anal and penile high-risk human papillomavirus (HPV) infection is associated
with anogenital cancer, which is especially common in HIV-infected MSM. We assessed …

Pectobacterium carotovorum subsp. carotovorum can cause potato blackleg in temperate climates

EG de Haan, TCEM Dekker-Nooren… - European Journal of …, 2008 - Springer
It is well established that the pectinolytic bacteria Pectobacterium atrosepticum (Pca) and
Dickeya spp. are causal organisms of blackleg in potato. In temperate climates, the role of …

Rapid and reliable discrimination between Shigella species and Escherichia coli using MALDI-TOF mass spectrometry

A Paauw, D Jonker, G Roeselers, JME Heng… - International Journal of …, 2015 - Elsevier
E. coli–Shigella species are a cryptic group of bacteria in which the Shigella species are
distributed within the phylogenetic tree of E. coli. The nomenclature is historically based and …

Shifts in the dominant populations of ammonia-oxidizing b-subclass Proteobacteria along the eutrophic Schelde estuary

MJM de Bie, AGCL Speksnijder, GA Kowalchuk… - Aquatic Microbial …, 2001 - int-res.com
The community structure of ammonia-oxidizing bacteria of the b-subclass Proteobacteria
was investigated with respect to environmental gradients along the Schelde, a eutrophic …

Recreational drug use during sex and sexually transmitted infections among clients of a city sexually transmitted infections clinic in Amsterdam, the Netherlands

M Heiligenberg, PR Wermeling… - Sexually transmitted …, 2012 - journals.lww.com
Background: Recreational drug use is associated with high-risk sexual behavior and
sexually transmitted infections (STIs). We assessed the prevalence of drug use during sex …

[HTML][HTML] The effects of spatial and temporal replicate sampling on eDNA metabarcoding

KK Beentjes, AGCL Speksnijder, M Schilthuizen… - PeerJ, 2019 - peerj.com
Background The heterogeneous nature of environmental DNA (eDNA) and its effects on
species detection and community composition estimates has been highlighted in several …

The effect of HIV infection on anal and penile human papillomavirus incidence and clearance: a cohort study among MSM

SH Mooij, DK Van Santen, RB Geskus… - Aids, 2016 - journals.lww.com
Objectives: A large portion of anogenital cancers is caused by high-risk human
papillomavirus (hrHPV) infections, which are especially common in HIV-infected men. We …